Time-dependent expression of CD25 in phytohemagglutinin- or interleukin-2-stimulated human peripheral blood lymphocytes |
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Authors: | V V Zenin N D Aksenov A N Shatrova I I Marakhova |
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Institution: | (1) GASR Biotechnology, Kfar-Saba, 44395, Israel;(2) Frankel Laboratory, Center for Stem Cell Research, Schneider Children’s Medical Center of Israel, 14 Kaplan Street, Petach Tikva, Israel, 49202;(3) Minimally Invasive Surgical Technology Institute, Cedars Sinai Medical Center, Los Angeles, CA, USA; |
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Abstract: | The dynamics of the expression of the high-affinity receptor for interleukin-2 (IL-2 receptor) evaluated by the method of
flow cytofluorimetry based on changes in the number of cells that express the CD25 marker (CD25+) was studied in human peripheral
blood lymphocytes stimulated by various mitogens. It has been shown that, in the resting lymphocyte culture, both phytohemagglutinin
(PHA, 10 μg/ml) and 12,13-phorbol dibutyrate (PDBu, 10−8 M) with ionomycin (IM, 5 × 10−7 M) induce a long-lasting increase (for 48 h) in the number of CD25+ cells. Interleukin-2 (IL-2) has only been found to be
capable of inducing time-dependent CD25 expression in competent (not resting) lymphocytes pretreated with submitogenic doses
of PHA (1 μg/ml). A comparison of the dynamics of the number of CD25+ cells and blast transformation has shown that CD25 markers
are revealed as early as on small stimulated lymphocytes, while, at the late activation stages, which correspond to the stage
of cell growth and transition to DNA synthesis, the overwhelming majority of blasts are CD25+ cells with high-affinity α -receptors
for IL-2. The obtained data allow one to suggest that the expression of an α -subunit of IL-2 receptor takes place at the
IL-2-dependent stage of T lymphocyte proliferation and may be directly induced by IL-2 via IL-2 receptor. |
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