A组轮状病毒NSP6蛋白表达及免疫学性质研究

轮状病毒(RV）NSP6与NSP5由同一基因片段编码，至今对NSP6 性质了解很少。用基因重组表达和免疫学方法，重组表达了A组人RV NSP6蛋白，进行了NSP6的动物免疫及其抗原反应性、免疫原性研究以及RV感染细胞中NSP6的合成及亚细胞分布研究。研究结果表明，NSP6可在原核系统中高效表达，表达蛋白占菌体总蛋白的34.2%；NSP6免疫豚鼠血清抗体可特异性识别菌体细胞中表达的NSP6和SA11及Wa病毒感染的MA104细胞中合成的NSP6蛋白；病毒感染细胞中合成的NSP6在感染后3h就可检测到，12h表达量达到最高；NSP6在病毒感染细胞质中呈弥散状分布，并主要积聚在细胞核的周围，未观察到毒质体样结构。研究结果对深入了解RV NSP6的结构与功能具有重要的意义，具有重要的潜在应用价值。

Prokaryotic Expression and Immunological Reactivity of Rotavirus NSP6

Little is known about the non-structural protein 6(NSP6)of mtavirus.This report describes expression of the NSP6 of a group A human mtavims strain TB-Chen in bacteria,and its immunological properties and cellular distribution.The results showed that the recombinant NSP6(rNSP6)was expressed in high efficiency without any other proteins fused(possesses about 34.2%of total bacterial proteins).rNSP6 elicited mono-specific antibodies in immunized guinea pigs and the antibodies could react with the rNSP6 itself and the viral NSP6 proteins synthesized in SA11-or Wa4nfected MA104 cells in Western blot and immunofluorescence assay.The NSP6 distributed evenly in the cytoplasm mainly around the nucleus of virus-infected cells,no viroplasm-like gathering observed;The top amount of NSP6 synthesized in SA11-infected cells or Wa-infected cells could be detected at 12h after infection.This is the first report about the high expression of entire NSP6(without any other proteins fused)in prokaryotic expression system and detection of NSP6 synthesis in virus infected cells by immunofluorescence assay.The results are important to understand the structure.biological properties and further application of the NSP6.