Anti-galactocerebroside antibodiesin human cerebrospinal fluids determined by enzyme-linked immunosorbent assay (ELISA) |
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| Authors: | Takao Ichioka Ken-ichiro Uobe Michael Stoskopf Yasuo Kishimoto PhD Gihan Tennekoon Wallace W Tourtellotte |
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| Institution: | (1) The Kennedy Institute, School of Medicine, Johns Hopkins University, 707 North Broadway, 21205 Baltimore, Maryland;(2) Department of Neurology School of Medicine, Johns Hopkins University, 21205 Baltimore, Maryland;(3) Department of Contparative Medicine, School of Medicine, Johns Hopkins University, 21205 Baltimore, Maryland;(4) Neurology Service Veterans Administration, Wadsworth Medical Center and Department of Neurology, University of California, 90073 Los Angeles, California;(5) Present address: Department of Pediatrics, Tokushima University School of Medicine, 770 Tokushima, Japan;(6) Present address: Department of Oral Pathology, Osaka Dental University, 540 Osaka, Japan |
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| Abstract: | The standard ELISA technique was improved for the detection of antigalactocerebroside antibody in biological fluid. Mouse monoclonal antigalactocerebroside antibody was used to demonstrate specificity and sensitivity of the technique. After optimization of the assay, the usefulness of this measurement for the evaluation of patients with multiple sclerosis was assessed. The presence of antigalactocerebroside antibodies in the cerebrospinal fluid of 20 patients with multiple sclerosis, 10 with other neurological diseases and 10 normal individuals was determined. All the CSF samples from normal individuals were negative. In patients with multiple sclerosis 14 of the 20 samples had elevated levels of antigalactocerebroside antibody, whereas with other neurological diseases 5 out of 10 were positive. Antigalactocerebroside levels were lower in samples from patients during an acute relapse than in those from more chronic cases. These results indicate that the presence of anti-galactocerebroside antibody in cerebrospinal fluid is not specific to MS but may reflect previous damage to myelin.Abbreviations and trivial names used ELISA
Enzyme-Linked Immunosorbent Assay
- CSF
cerebrospinal fluid; galacto- or glucocerebroside, ceramide-1-0-beta-galactoside or-glucoside |
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| Keywords: | Antibody myelin cerebroside multiple sclerosis ELISA cerebrospinal fluid |
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