Undifferentiated F9 embryonal carcinoma cells produce a short-chain collagen molecule. |
| |
| Authors: | B D Smith and C T Baldwin |
| |
| Institution: | Collagen Research Laboratory, Veterans Administration Outpatient Clinic, Boston, MA 02108. |
| |
| Abstract: | The undifferentiated F9 embryonal carcinoma cells produce a unique collagen that decreases in amount during retinoic acid-induced differentiation of F9 cells into basement-membrane parietal endoderm. A bacterial-collagenase-sensitive protein of approx. 60,000 Da was resolved on polyacrylamide-gel electrophoresis. After pepsin digestion, two pepsin-resistant fragments containing hydroxyproline were demonstrated, suggesting that a portion of the molecule has a stable triple helix. The mRNA from the undifferentiated F9 cells translates a collagenase-sensitive protein with a molecular mass consistent with the 60,000 Da collagenous protein produced by undifferentiated F8 cells. |
| |
| Keywords: | |
|
|
