Biological control of rice sheath blight using hyphae-associated bacteria: development of an <Emphasis Type="Italic">in planta</Emphasis> screening assay to predict biological control agent performance under field conditions |
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Authors: | Mette Haubjerg Nicolaisen Nguyen Duc Cuong Jakob Herschend Birgit Jensen Le Cam Loan Pham Van Du Jan Sørensen Helle Sørensen Stefan Olsson |
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Institution: | 1.Section of Microbial Ecology and Biotechnology, Department of Plant and Environmental Sciences, Faculty of Sciences,University of Copenhagen,Frederiksberg C,Denmark;2.Department of Plant Pathology,Cuu Long Rice Research Institute,Can Tho City,Vietnam;3.Data Science Lab, Department of Mathematical Sciences,University of Copenhagen,Copenhagen East,Denmark;4.Section of Microbiology, Department of Biology, Faculty of Sciences,University of Copenhagen,K?benhavn ?,Denmark;5.State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou,China |
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Abstract: | A pathogenicity assay for in planta screening of biological control agents (BCAs) towards sheath blight in rice was developed, and used to evaluate a panel of Rhizoctonia solani Kühn hyphae-associated bacteria for their ability to control sheath blight under screenhouse conditions. The best performing BCA, Burkholderia sp. strain A-7.3, was selected for field trials. Disease incidence and disease severity were significantly reduced leading to a significant grain yield increase of 7%. Furthermore, R. solani sclerotia formation and sclerotia viability were significantly reduced, thus lowering the reservoir for primary infections in the next crop cycle. The paper presents a reliable pathogenicity assay that can be used to test BCA performance under different scenarios e.g. plant variety and soil conditions, and help predict translation of results obtained under screenhouse conditions to field performance. Furthermore, it supports the hypothesis that hyphae-associated bacteria are a promising source of niche-specific BCAs towards fungal pathogens. |
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