High affinity binding sites for somatostatin to rat pituitary |
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Authors: | J C Reubi M Perrin J Rivier W Vale |
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Affiliation: | The Salk Institute, P. O. Box 85800, San Diego, California 92138 USA |
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Abstract: | Binding sites for somatostatin (SS) are described in rat pituitary membranes using either [125I-Tyr11]-SS-14 or [Leu8, D-Trp22, 125I-Tyr25]-SS-28 as radioligands; in each case saturable and high affinity binding sites with KD's for SS of 1.09 and 0.95 nM respectively have been characterized. The binding capacity is 100 f mols/mg protein. The potencies of various SS analogs measured in the radioreceptor assay are in agreement with the potencies in a bioassay measuring inhibition of growth hormone release; in particular, SS-28 is slightly less potent than SS-14. A comparison of these data with those describing SS binding in brain and pancreas suggests that some pharmacological differences may exist between pituitary, brain and pancreas binding sites for SS. |
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Keywords: | SS somatostatin GH growth hormone TSH thyrotropin-stimulating-hormone GnRH gonadotropin-releasing hormone CRF corticotropin-releasing factor CNS central nervous system HPLC high-pressure liquid chromatography |
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