Bioremediation 2,4,6,-Trichlorophenol (2,4,6-TCP) by Shigella sp. S2 Isolated from Industrial Dumpsite |
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Authors: | Santosh Kr Karn Sanjeev Balda |
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Institution: | Department of Biotechnology Engineering , Ambala College of Engineering and Applied Research , Haryana , India |
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Abstract: | A bacterium that utilizes 2,4,6-trichlorophenol (2,4,6-TCP) as a sole source of carbon and energy was isolated from an industrial dumpsite, the bacterium designated as strain S2. Degradation was routinely monitored by observing growth analysis, chloride release assay, and ring cleavage activity and was further confirmed by gas chromatography (GC) analysis. The bacterium was found to degrade up to 90% of 2,4,6-TCP at 1.5 mM concentration. The bacteria were characterized morphologically, biochemically, and by 16S rRNA gene sequencing, which showed 99% sequence similarity with Shigella sp. This is the first report that Shigella sp. was able to degrade 2,4,6-TCP. This strain was found to be novel and a potential 2,4,6-TCP degrader. Further, this strain may be used for bioremediation of 2,4,6-TCP–containing waste in the environment. |
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Keywords: | 16S rRNA gene sequence bioremediation GC analysis industrial sludge Shigella sp 2 4 6-trichlorophenol |
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