Photosynthesis in Rhodospirillum rubrum. I. Autotrophic Carbon Dioxide Fixation |
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Authors: | Louise Anderson and R C Fuller |
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Institution: | Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755 |
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Abstract: | The incorporation and distribution of activity from 14CO2 was investigated under autotrophic conditions in the facultative photoautotroph, Rhodospirillum rubrum, with cells cultured on hydrogen, carbon dioxide, and ammonium sulfate. In 1 second 14CO2 fixation experiments essentially all of the activity was found in 3-phosphoglyceric acid: plotted against time percent incorporation into phosphate esters has a strikingly negative slope. These results suggest that under autotrophic conditions the reductive pentose phosphate cycle or the key reactions of the cycle play a major role in carbon metabolism in this photosynthetic bacterium. Incorporation into amino acids and into intermediates of the tricarboxylic acid cycle was quite low. |
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