Effects of retinoic acid on the growth of cultured rabbit articular chondrocytes: Relation with alkaline phosphatase activity and beta receptor |
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| Authors: | Nathalie Dronne Laurent Benel Sophie Thenet Suzanne Larno Etienne Mokondjimobe Richard Bourbouze Monique Adolphe |
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| Institution: | (1) Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, Institut Biomédical des Cordeliers, 15, Rue de l'Ecole de Médecine, 75006 Paris, France;(2) Laboratoire de Chimie Biologique U.E.R. de Biologie Humaine et Expérimentale, Université René Descartes, 4, Avenue de l'Observatoire, 75006 Paris, France |
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| Abstract: | The effects of retinoic acid (RA) on rabbit articular cartilage cells were studied for concentrations ranging from 5.10-5 M to 10-7 M; the treatment with RA over three days resulted in dose dependent inhibition of chondrocyte proliferation between 5.10-5 and 10-5 M with persistence of the inhibitory effect until 10-6 M. RA until 10-7 M induced a slight, but significant, enhancement of cell proliferation. This growth stimulating effect seems to be related to the Beta receptor system because Beta blockers, such as sotalol and DL propranolol, were able to suppress the stimulating action of agonist type isoprenaline. The activity of alkaline phosphatase (AP) was also determined. The highest dose of RA (5.10-5 M) induced an increase (x 3) of AP activity, and 10-7 M RA decreased it (x 0.4). |
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| Keywords: | rabbit articular chondrocytes retinoic acid  -blocker" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-blocker alkaline phosphatase |
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