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Expression profile of BDNF-responsive genes during cerebellar granule cell development
Authors:Sato Masaaki  Suzuki Kazunori  Nakanishi Shigetada
Institution:Department of Molecular and Systems Biology, Graduate School of Biostudies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
Abstract:With the aid of microarray and PCR analysis, this investigation sought expression profiles of BDNF-regulated genes in cultured mouse cerebellar granule cells and addressed their relevance to gene regulation in developing granule cells in vivo. Many of the BDNF-upregulated and downregulated genes identified were upregulated and downregulated, respectively, during cerebellar development. This developmental change was, at least partly, prevented in the TrkB receptor-deficient cerebellum. The BDNF-upregulated genes were distributed in either postmigratory or both premigratory and postmigratory granule cells at postnatal day 8 (P8) and were still present in mature granule cells at P21. In contrast, the BDNF-downregulated genes were predominantly expressed in premigratory granule cells at P8 and disappeared at P21. Furthermore, many of the BDNF-upregulated gene products are implicated in signaling cascades of N-methyl-D-aspartate receptors and MAP kinase. The results indicate that BDNF signaling plays a pivotal role in promoting gene expression in granule cell development and maturation.
Keywords:Cerebellum  Granule cell  Development  Primary culture  Microarray  Gene expression profile  Brain-derived neurotrophic factor  d-aspartate receptor" target="_blank">N-Methyl-d-aspartate receptor  TrkB receptor
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