Oxidized low density lipoprotein inhibits the hemolytic activity of Asp-hemolysin from Aspergillus fumigatus |
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| Authors: | Yuji Fukuchi Yoichi Kudo Takeshi Kumagai Keiichi Ebina Katsushi Yokota |
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| Affiliation: | Japanese Red Cross Sendai Hospital, 2-43-3, Yagiyamahon-cho, Taihaku-ku, Sendai 9828501, Japan;Sendai Hospital of East Japan Railway Company, 1-3-1, Itsutsubashi, Aoba-ku 9808508, Japan;The First Department of Hygienic Chemistry, Tohoku College of Pharmacy, 4-4-1, Komatsusima, Aoba-ku, Sendai 9818558, Japan |
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| Abstract: | We have examined the effect of chemically modified human low density lipoproteins (LDLs) , acetylated LDL and oxidized LDL, on the hemolytic activity of Asp-hemolysin. Oxidized LDL, but not acetylated LDL, inhibited the hemolytic activity of this toxin. The inhibitory effects of oxidized LDL increased with the time of Cu2+-induced LDL oxidation. Similar inhibition was observed in the filtrate which was separated from the incubation mixture of Asp-hemolysin with oxidized LDL (for 2 h of oxidation) following ultrafiltration through a membrane with a molecular mass cutoff of 100 000. However, at longer LDL oxidation times, the inhibition by the filtrates was less than the control mixture without ultrafiltration. We suggest that the inhibition by oxidized LDL was due to the binding of oxidized LDL to Asp-hemolysin at shorter LDL oxidation times . |
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| Keywords: | Asp-hemolysin Hemolytic activity Low-density lipoprotein Oxidized low-density lipoprotein |
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