The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron |
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Authors: | Reiber Kathrin Reeves Emer P Neville Claire M Winkler Robert Gebhardt Peter Kavanagh Kevin Doyle Sean |
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Affiliation: | National Institute for Cellular Biotechnology, Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland. |
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Abstract: | Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD ( approximately 200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy. |
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Keywords: | NRPS Iron Siderophore MALDI ToF Secondary metabolites Proteomics 4'-PPTase |
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