Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis |
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| Authors: | Portillo M C Villahermosa D Corzo A Gonzalez J M |
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| Institution: | Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Seville, Spain. |
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| Abstract: | Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison. |
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