Carbon monoxide sensitivity of cytochrome oxidase in wheat leaves in relation to dwarfing genes (Rht) in wheat cultivars |
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Authors: | M S Naik D J D Nicholas |
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Institution: | (1) Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064 Glen Osmond, South Australia |
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Abstract: | Summary Leaves of young seedlings of a number of tall cultivars of wheat, lacking the dwarfing Rht genes, readily responded to a brief 2 min exposure to CO, as assessed by in vivo aerobic assay of nitrate reductase. This test depends on the inhibition of cytochrome c oxidase by CO, which in turn renders cytosolic NADH available for the reduction of nitrate to nitrite in vivo. Semi-dwarf cultivars of wheat (Rht present) did not respond to CO in this way. Since CO forms a complex only with reduced cytochrome a3, the results indicate differences in the redox state of cytochrome a3, during in situ respiration of leaves from tall and semi-dwarf plants which are likely to be under genetic control. |
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Keywords: | Rht genes Cytochrome oxidase CO effects Triticum aestivum L |
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