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Analysis of the pattern of gene expression during human adipogenesis by DNA microarray |
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| Authors: | Lubing Zhou Yuan-Di Halvorsen Ellen V Cryan Patricia D Pelton Thomas P Burris Keith T Demarest |
| Institution: | (1) Endocrine Therapeutics, Drug Discovery, The R.W. Johnson Pharmaceutical Research Institute, 1000 Route 202, South Raritan, NJ 08869, USA;(2) Zen Bio, Inc., Research Triangle Park, NC 27709, USA |
| Abstract: | High density DNA microarrays containing over 5000 cDNA clones were used to carry out a comprehensive investigation of gene expression during adipogenesis. Complex probes synthesized from total RNA were hybridized to the arrays to determine the level of mRNA expression of each arrayed gene. Thirty three genes (29 known and 4 ESTs with no identified homologies) have been found to alter their level of expression more than 2.5-fold after differentiation. The quantitative measurement by DNA array was in good agreement with conventional Northern blot analysis of selected genes. Our results demonstrate that utilization of a DNA array is a speedy, efficient and quantitative approach to profile the expression of a large number of genes. |
| Keywords: | adipocyte differentiation DNA chip PPAR |
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