| 4C-克隆筛选过程中的质量控制 |
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| 引用本文： | Tian J,Chen N,Zhao ZH,Chen HP. 4C-克隆筛选过程中的质量控制[J]. 遗传, 2011, 33(4): 404-410. DOI: 10.3724/SP.J.1005.2011.00404 |
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| 作者姓名： | Tian J Chen N Zhao ZH Chen HP |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
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| 摘 要： | 环形染色体构象俘获(Circular chromosome conformation capture,4C)是一种高通量研究细胞染色体相互作用、空间构象的技术。文章通过模拟的4C样品,优化反向PCR条件,建立了高效、特异的扩增方法,对4C克隆筛选等后续步骤进行严格的质量控制并对该方法的可行性进行了实例验证。该4C-克隆筛选方法作为4C方法的质量控制标准,具有重要的指导和监测作用。
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| 关 键 词： | 环形染色体构象俘获 染色体相互作用 质量控制 SOX2 PCR扩增 |
The quality control of 4C-clone screening assay |
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| Tian Jing,Chen Na,Zhao Zhi-Hu,Chen Hui-Peng. The quality control of 4C-clone screening assay[J]. Hereditas, 2011, 33(4): 404-410. DOI: 10.3724/SP.J.1005.2011.00404 |
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| Authors: | Tian Jing Chen Na Zhao Zhi-Hu Chen Hui-Peng |
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| Affiliation: | TIAN Jing,CHEN Na,ZHAO Zhi-Hu,CHEN Hui-Peng Institute of Biotechnology,Academy of Military Medical Science,Beijing 100071,China |
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| Abstract: | Circular chromosome conformation capture(4C) can be used to analyze the high-resolution interaction map of cis-regulatory elements in genome-wide studies.In this study,we optimized the condition of PCR with the mimical 4C sample in order to establish a specific and efficient assay,which was validated practically.This 4C-clone screening assay can be used as the quality control in the application of 4C assay. |
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| Keywords: | SOX2 |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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