| 梅花鹿鹿茸尖端组织ESTs分析 |
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| 引用本文： | Hao L,Li HP,Yan L. 梅花鹿鹿茸尖端组织ESTs分析[J]. 遗传, 2011, 33(4): 371-377. DOI: 10.3724/SP.J.1005.2011.00371 |
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| 作者姓名： | Hao L Li HP Yan L |
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| 作者单位： | 东北林业大学野生动物资源学院,哈尔滨,150040 |
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| 基金项目： | 黑龙江省自然科学基金项目,中央高校基本科研业务费专项资金项目 |
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| 摘 要： | 文章通过对东北梅花鹿(Cervus nippon hortulorum)鹿茸尖端组织cDNA文库随机测序获得了906条高质量ESTs,906条ESTs拼接后代表了701个Unigenes,其中包括重叠群86个,单拷贝615个。Blast分析显示具已知和推测功能的基因580个(82.7%),通过Gene Ontology(GO)分类对获得的580个功能基因进行了包括分子功能、生物过程和细胞组分在内的3个层次的功能注释,并根据BLAST的注释结果及进一步的筛选与分析,共得到39条与鹿茸尖端组织生长发育相关的基因。cDNA文库的构建和ESTs分析填补了鹿科动物在NCBI公共数据库上基因组信息的空白,并为科学的开发和利用梅花鹿资源提供了重要的理论依据。
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| 关 键 词： | 梅花鹿 鹿茸 cDNA文库 表达序列标签(ESTs) 基因本体论 |
Analysis of expressed sequence tags (ESTs) in sika deer (Cervus nippon hortulorum) velvet tip tissue |
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| Hao Li,Li He-Ping,Yan Li. Analysis of expressed sequence tags (ESTs) in sika deer (Cervus nippon hortulorum) velvet tip tissue[J]. Hereditas, 2011, 33(4): 371-377. DOI: 10.3724/SP.J.1005.2011.00371 |
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| Authors: | Hao Li Li He-Ping Yan Li |
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| Affiliation: | HAO Li,LI He-Ping,YAN Li College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China |
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| Abstract: | A total of 906 high quality ESTs were obtained through random sequencing from velvet tip tissue cDNA library of Sika Deer(Cervus nippon hortulorum).Clustering of 906 ESTs resulted in a set of 701 unigenes comprising of 86 con-tigs and 615 singletons.BLAST analysis revealed that 580 unigenes(82.7% of all 701 unigenes) were matched to the known genes or putative genes in public databases,which could be classified into molecular function,biological process or cellular component according to the Gene Ontology(G... |
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| Keywords: | sika deer velvet cDNA library expressed sequence tags(ESTs) Gene Ontology(GO) |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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