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| 新基因BM390716、BI274487和AA963863与大鼠再生肝8种细胞的细胞外基质代谢 | |
| 作者姓名： | Wang Z Wen YY Cheng ZC Guo XQ Zhang XS Xu CS |
| 作者单位： | 1. 河南师范大学生命科学学院,新乡,453007;河南省一科技部共建细胞分化调控国家重点实验室培育基地,新乡,453007 2. 河南师范大学生命科学学院,新乡,453007 3. 河南省一科技部共建细胞分化调控国家重点实验室培育基地,新乡,453007 |
| 基金项目： | 国家973项目前期研究专项 |
| 摘 要： | 为了解新基因BM390716、BI274487、AA963863在细胞外基质代谢中的作用及其与大鼠肝再生的相关性,文章用Percoll密度梯度离心结合免疫磁珠分选分离大鼠再生肝的8种细胞,用Rat Genome 230 2.0芯片检测它们的基因表达变化,用Microsoft Excel、BLAST等软件分析基因的共表达关系、序列同源性及参与的代谢活动。结果表明,BM390716与pparα同源和共表达,BI274487与timp2同源和共表达,AA963863与csgalnact1同源和共表达。根据上述基因的同源性和共表达推测,新基因BM390716、BI274487和AA963863参与大鼠再生肝8种细胞的细胞外基质代谢。 |
| 关 键 词： | 大鼠肝再生 Rat Genome2302.0芯片 基因的同源性和共表达 细胞外基质代谢 |
Three novel genes BM390716, BI274487 and AA963863 involed in extracellular matrix metabolism of eight rat regenerating liver cell types |
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| Wang Z,Wen YY,Cheng ZC,Guo XQ,Zhang XS,Xu CS.Three novel genes BM390716, BI274487 and AA963863 involed in extracellular matrix metabolism of eight rat regenerating liver cell types[J].Hereditas,2011,33(4):378-388. | |
| Authors: | Wang Ze Wen Yuan-Yuan Cheng Zhen-Chao Guo Xue-Qiang Zhang Xin-Sheng Xu Cun-Shuan |
| Institution: | WANG Ze1,2,WEN Yuan-Yuan1,CHENG Zhen-Chao1,GUO Xue-Qiang2,ZHANG Xin-Sheng1,XU Cun-Shuan1,2 1.College of Life Science,Henan Normal University,Xinxiang 453007,China,2.Key Laboratory for Cell Differentiation Regulation |
| Abstract: | To explore the roles of three novel genes BM390716,BI274487 and AA963863 and the correlation between them during liver regeneration of rats,eight kinds of liver cells were isolated using the combined percoll density gradient centrifugation and immunomagnetic bead method.Rat genome 230 2.0 array was used to detect the changes in expression of genes involved in metabolism of extracellular matrix and the novel genes in rat genome.Correlation between sequence homology,co-expression of the above genes and the ph... |
| Keywords: | liver regeneration Rat Genome 230 2 0 array gene homology and co-expression extracellular matrix metabolism |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |