Glycosphingolipids of Human Peripheral Nervous System Myelins Isolated from Cauda Equina |
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| Authors: | K Ogawa-Goto Y Ohta K Kubota N Funamoto T Abe T Taki K Nagashima† |
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| Institution: | Department of Pediatrics, Teikyo University School of Medicine, Tokyo, Japan;Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, Tokyo, Japan;Department of Pathology, School of Medicine, Hokkaido University, Sapporo, Japan |
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| Abstract: | Abstract: Compositions of neutral and sulfated glucuronyl glycosphingolipids purified from human motor and sensory nerves and myelins were studied. Higher neutral glycosphingolipids (fraction B), which were separated from GazlCer (fraction A), were analyzed by TLC and TLC-immunostaining. Both nerve myelins contained paragloboside (nLc4Cer) and nLc6Cer dominantly as major higher glycosphingolipids and very little globoside (Gb4Cer), whereas both nerves contained Gb4Cer and nLc4Cer. Besides these major glycosphingolipids, a neutral glycolipid containing asialoGMI (Gg4Cer) epitope and other minor components such as ceramide trihexoside and ceramide dihexoside were detected in both nerves and their myelins. Furthermore, sulfated glucuronyl nLc4Cer and n Lc6Cer, which were monoclonal antibody HNK-1 reactive glycolipids, were detected in both nerves and myelins. |
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| Keywords: | Myelin Glycosphingolipids Peripheral nervous system Sulfated glucuronyl glycosphingolipids Asialo GMI Paragloboside |
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