Evaluation of sugar cane hemicellulose hydrolyzate for cultivation of yeasts and filamentous fungi |
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Authors: | A Pessoa Jr I M de Mancilha S Sato |
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Affiliation: | Departamento de Biotecnologia/Faenquil, 12.600-000, Lorena/SP, Brazil, BR Biochemical and Pharmaceutical Dept/FCF/USP-POB 66083, S?o Paulo/SP, Brazil, BR
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Abstract: | Sugar cane bagasse hemicellulosic fraction submitted to hydrolytic treatment with 100 mg of sulfuric acid per gram of dry mass, at 140°C for 20 min, was employed as a substrate for microbial protein production. Among the 22 species of microorganisms evaluated, Candida tropicalis IZ 1824 showed TRS consumption rate of 89.8%, net cell mass of 11.8 g L−1 and yield coefficient (Yx/s) of 0.50 g g−1. The hydrolyzate supplemented with rice bran (20.0 g L−1), P2O5 (2.0 g L−1) and urea (2.0 g L−1) provided a TRS consumption rate of 86.3% and a cell mass of 8.4 g L−1. At pH 4.0 cellular metabolism was inhibited, whereas at pH 6.0 the highest yield was obtained. The presence of furfural (2.0 g L−1) hydroxymethylfurfural (0.08 g L−1) and acetic acid (3.7 g L−1) in the hydrolyzate did not interfere with cultivation at pH 6.0. Received 25 October 1996/ Accepted in revised form 10 March 1997 |
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Keywords: | : xylose hemicellulosic hydrolyzate sugar-cane-bagasse microbial protein |
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