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Analysis of Pteridium ribosomal RNA sequences by rapid direct sequencing
Authors:Mui Keng Tan
Affiliation:(1) School of Biological Sciences, University of Sydney, 2006, NSW, Australia;(2) Present address: Biological and Chemical Research Institute, NSW Department of Agriculture, PMB 10, 2116 Rydalmere, NSW, Australia
Abstract:A total of 864 bases from 5 regions interspersed in the 18S and 26S rRNA molecules from various clones of Pteridium covering the general geographical distribution of the genus was analysed using a rapid rRNA sequencing technique. No base difference has been detected amongst the three major lineages, two of which apparently separated before the breakup of the ancient supercontinent, Pangaea. These regions of the rRNA sequences have thus been conserved for at least 160 million years and are here compared with other eukaryotic, especially plant rRNAs.
Keywords:Pteridium  18S rRNA  26S rRNA  sequencing
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