Sugar uptake in a 2-deoxy-d-glucose resistant mutant ofSaccharomyces cerevisiae |
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Authors: | Srdjan Novak Tony D'Amore Inge Russell Graham G Stewart |
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Institution: | (1) Research Department, Labatt Brewing Company Limited, 150 Simcoe Street, N6A 4M3 London, Ontario, Canada;(2) Present address: Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Yugoslavia |
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Abstract: | Summary The non-metabolizable and toxic glucose analogue 2-deoxy-d-glucose (2-DOG) has been widely employed to screen for regulatory mutants which lack catabolite repression. A number of yeast mutants resistant to 2-DOG have recently been isolated in this laboratory. One such mutant, derived from aSaccharomyces cerevisiae haploid strain, was demonstrated to be derepressed for maltose, galactose and sucrose uptake. Furthermore, kinetic analysis of glucose transport suggested that the high affinity glucose transport system was also derepressed in the mutant strain. In addition, the mutant had an increased intracellular concentration of trehalose relative to the parental strain. These results indicate that the 2-DOG resistant mutant is defective in general glucose repression. |
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Keywords: | d-glucose" target="_blank">2-Deoxy-d-glucose Yeast Catabolite repression Derepression |
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