Characterization of minute differences between genomes of strains of Penicillium nalgiovense using subtractive suppression hybridization without cloning

AIMS: Characterization of integrated DNA fragments of unknown base sequences from foreign species using the genetically modified mould Penicillium nalgiovense. METHODS AND RESULTS: A modified suppression subtractive hybridization method was deployed in order to detect and characterize foreign genes. Combined with a special two-dimensional polyacrylamide gel electrophoretic technique the usual background was eliminated from the prospective foreign polymerase chain reaction fragments according to their base composition (Müller et al. 1981). CONCLUSIONS AND SIGNIFICANCE AND IMPACT OF THE STUDY: DNA fragments obtained from the procedure were suitable for direct DNA sequencing. Each DNA fragment derived from the tester genomic DNA was found to be completely absent in the driver organism.