| The Effect of Bovine IFN-α on the Immune Response in Guinea Pigs Vaccinatedwith DNA Vaccine of Foot-and-Mouth Disease Virus |
| |
| 引用本文： | Guo HC,Liu ZX,Sun SQ,Leng QW,Li D,Liu XT,Xie QG. The Effect of Bovine IFN-α on the Immune Response in Guinea Pigs Vaccinatedwith DNA Vaccine of Foot-and-Mouth Disease Virus[J]. Acta biochimica et biophysica Sinica, 2004, 36(10): 701-706 |
| |
| 作者姓名： | Guo HC Liu ZX Sun SQ Leng QW Li D Liu XT Xie QG |
| |
| 作者单位： | Hui-Chen GUO,Zai-Xin LIU*,Shi-Qi SUN,Qing-Wen LENG1,Dong LI,Xiang-Tao LIU,and Qing-Ge XIE* Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China; College of Animal Science and Technology,Shihezi University,Shihezi 832003,China 1 |
| |
| 基金项目： | This work was supported by a grant from the Major State BasicResearch Development Program of China (No. G1999011903) |
| |
| 摘 要： | Foot-and-mouth disease virus (FMDV) belongs to thegenus Aphthovirus of the family Picornavidae. The FMDVgenome is a copy of positive-sense, single-stranded RNA,which contains one large open reading frame (ORF). TheORF is translated into a polypeptide, which undergoesautoproteolytic cleavage to produce the structural and non-structural proteins and ultimately forms mature viral pro-teins [1,2]. FMD is caused by the FMDV, which is a highly conta-gious vesicular disease of cloven-hoofe…
|
| 关 键 词： | foot-and-mouth disease virus (FMDV) DNA vaccine IFN-α gene vaccination |
The effect of bovine IFN-alpha on the immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus |
| |
| Guo Hui-Chen,Liu Zai-Xin,Sun Shi-Qi,Leng Qing-Wen,Li Dong,Liu Xiang-Tao,Xie Qing-Ge. The effect of bovine IFN-alpha on the immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus[J]. Acta biochimica et biophysica Sinica, 2004, 36(10): 701-706 |
| |
| Authors: | Guo Hui-Chen Liu Zai-Xin Sun Shi-Qi Leng Qing-Wen Li Dong Liu Xiang-Tao Xie Qing-Ge |
| |
| Affiliation: | Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. |
| |
| Abstract: | In this study, we constructed recombinant plasmid pcDNA3.1/P12X3C3D including P1, 2A, 3C, 3D and part of 2B gene of FMDV and pcDNA3.1/IFN containing the gene encoding bovine IFN-alpha. We inoculated the DNA vaccine pcDNA3.1/P12X3C3D with or without pcDNA3.1/IFN to evaluate the efficiency of this DNA vaccine and the immunogenicity of DNA vaccine enhanced by the co-delivery with pcDNA3.1/IFN. After two times of vaccination with DNA vaccine, all of guinea pigs were challenged with 103 ID50 FMDV type O. Anti-FMDV antibody levels were detected by ELISA and T lymphocyte proliferation response was tested by MTT assay. The result shows that guinea pigs inoculated by pcDNA3.1/P12X3C3D alone or with pcDNA3.1/IFN generated specific antibodies and induced an FMDV-specific T lymphocyte proliferation response. FMDV challenge tests showed that one in four guinea pigs immunized by pcDNA3.1/P12X3C3D with pcDNA3.1/IFN was protected from the FMDV serotype O infection. This result indicated that the efficiency of the DNA vaccine was enhanced by co-delivery with pcDNA3.1/IFN. However, the protection rate was considerably lower than that immunized with conventional FMD vaccine. |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 PubMed 等数据库收录！ |
|
