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Restriction map of the temperate phage E105 from the polylysogenic culture of Erwinia carotovora 268
Authors:Iu A Grigorian  F I Tovkach  V V Danile?chenko  Ia G Kishko
Abstract:DNA structure of the temperate bacteriophage E105 from polylysogenic culture of Erwinia carotovora 268 has been studied. The viral 29.12-29.17 MD DNA has been shown to be linear and nonpermuted. The complete restriction map of the viral DNA has been constructed for MvaI and HpaI and partial for Eco31 restriction endonucleases based on the pair hydrolysis of the native DNA as well as its fragments. Altogether, 19 sites for restriction endonucleases have been localized on bacteriophage DNA.
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