Combined effects of niacin and chromium treatment on vascular endothelial dysfunction in hyperlipidemic rats |
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Authors: | N Niu Y H Yu Y Wang L J Wang Q Li L M Guo |
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Institution: | (1) Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China |
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Abstract: | Combined treatment with niacin and chromium has been found to have a protective effect against oxidative damage to different
tissues of hyperlipidemic rats. But its effects on vascular endothelial dysfunction are less clear. This study was performed
to investigate the effect of combined treatment with niacin and chromium on vascular endothelial dysfunction, with the aim
of gaining insight to the mechanisms by detecting the expression levels of ox-LDL and LOX-1. Twenty-four male, 4-week-old
Wistar rats were randomly divided into three groups: control group (CG; n = 8), high-fat group (HF; n = 8), and drug control group (DG; n = 8).In CG group, rats were fed with pellet chow. In HF group, rats were fed with high-fat diet for 12 weeks. In DG group,
rats were fed with the same high-fat diet and treated with CrCl3 · 6H2O (250 μg/kg days) and niacin (100 mg/kg days) by gavage technique for 12 weeks. At the end of the 12th week, samples from
aorta and blood were collected. In HF group, the serum levels of total cholesterol (TC), low-density lipoprotein (LDL), oxidized
low-density lipoprotein (ox-LDL) and endothelin (ET) were higher, whereas the levels of high-density lipoprotein (HDL), serum
NO were lower than those in CG group. The levels of serum TC, LDL, ox-LDL and ET decreased and HDL, NO levels increased in
DG group when compared with HF group. The levels of LOX-1, ICAM-1 were also observed in abdominal artery. In HF group, the
protein and mRNA expression of LOX-1, ICAM-1 were elevated comparing with CG group. In DG group, the protein and mRNA expression
of LOX-1, ICAM-1 were decreased obviously, but still differed significantly from those in CG group. ox-LDL was related positively
to TC, LDL, ET, ICAM-1 and LOX-1, but related negatively to NO and HDL. These findings indicated that combined treatment with
niacin and chromium has potential therapeutic protection of endothelial function by down-regulating ox-LDL/LOX-1 signaling pathway. |
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Keywords: | Niacin Chromium Endothelial dysfunction Receptor LDL |
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