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| 大豆种质对SMV成株和种粒斑驳抗性的SSR标记辅助鉴定 | |
| 引用本文： | 李文福,朱晓双,王晓锋,王堃,刘春燕,陈庆山,胡国华.大豆种质对SMV成株和种粒斑驳抗性的SSR标记辅助鉴定[J].植物遗传资源学报,2010,11(2):239-243. |
| 作者姓名： | 李文福 朱晓双 王晓锋 王堃 刘春燕 陈庆山 胡国华 |
| 作者单位： | 1. 黑龙江省农垦科研育种中心,哈尔滨,150090;东北农业大学农学院,哈尔滨,150030 2. 黑龙江省建三江农业科学研究所,富锦,156300 3. 黑龙江省农垦科研育种中心,哈尔滨,150090;国家大豆工程技术研究中心,哈尔滨,150050 |
| 基金项目： | 国家重点基础研究发展计划(973计划)项目，引进国际先进农业科学技术计划(948计划)项目(2006-G1，国家高技术研究发展计划(863计划)项目，国家自然科学基金 |
| 摘 要： | 对186份大豆种质资源进行了成株和种粒斑驳抗性鉴定，并利用与成株抗性及种粒斑驳抗性分别相关的SSR标记验证抗病毒分子辅助选择的可行性。结果表明：接种SMV1，选出成株和种粒双抗种质38份，成株抗病种质149份，种粒抗病种质45份，成株和种粒双感种质26份。利用与成株抗性相关的SSR标记Sat_229、Sat_317、Satt335、Satt160、Satt516、Sat_309进行检测，抗病毒资源筛选的准确率分别达到68．9％、74．3％、71．1％、69．8％、77．4％和68．2％。利用与种粒斑驳抗性相关的SSR标记Sat_297、Sat_229、Sat_317、Satt335、Set_188、Satt160、Satt516、Sat_133进行检测，Sat_317标记准确率达79．1％，标记Sat_229、Satt335、Satt516和Sat_133抗病毒资源筛选的准确率均达70％以上，可以用作抗病毒分子辅助育种的选择标记。 |
| 关 键 词： | 大豆花叶病毒 种质资源 种粒斑驳 分子标记 辅助选择 |
Identification of the SMV adult-plant and seed coat mottling resistance in soybean germplasms using SSR markers |
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| LI Wen-fu,ZHU Xiao-shuang,WANG Xiao-fong,WANG Kun,LIU Chun-yan,CHEN Qing-shan,HU Guo-hua.Identification of the SMV adult-plant and seed coat mottling resistance in soybean germplasms using SSR markers[J].Journal of Plant Genetic Resources,2010,11(2):239-243. | |
| Authors: | LI Wen-fu ZHU Xiao-shuang WANG Xiao-fong WANG Kun LIU Chun-yan CHEN Qing-shan HU Guo-hua |
| Institution: | LI Wen-fu~(1,2),ZHU Xiao-shuang~(1,WANG Xiao-feng~4,WANG Kun~(1,LIU Chun-yan~(1,CHEN Qing-shan~(1,HU Guo-hua~(1,3) (1 The Crop Research , Breeding Center of L,-Reclamation,Harbin 150090,2 College of Agriculture,Northeast Agricultural University,Harbin 150030,3 The National Research Center of Soybean Engineering , Technology,Harbin 150050,4 Jiansanjiang Institute of Agricultural Sciences in Heilongjiang,Fujin 156300) |
| Abstract: | One hundred and eighty six soybean germplasms were screened for resistance to adult-plant and seed coat mottling by the inoculation of strain SMV1,and the coincident rate between adult-plant resistance,seed coat mottling resistance to SMV and linkage SSR markers were evaluated.The results showed that among these 186 germplasms, 38 were resistant to both adult-plant and seed coat mottling;149 were only resistant to adult-plant;45 were only resistant to seed coat mottling;and 26 were both susceptible to adult... |
| Keywords: | Soybean mosaic virus Germplasm resources Seed coat mottling Molecular markers Assisted selection |
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