An enzyme-amplified diffraction-based immunoassay |
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Authors: | Loo Richard W Tam Pui L Goh Jane Betty Goh M Cynthia |
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Institution: | Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Canada M5S 3H6. rloo@chem.utoronto.ca |
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Abstract: | We have previously shown that a gold-conjugated secondary label can be used to reduce the limit of detection in a diffraction-based assay by more than 40-fold. We now show that by using a combination of a peroxidase-conjugated secondary label and a precipitating substrate the limit of detection in a diffraction-based assay can be reduced by more than 1000-fold. The response to secondary enhancement was linear for concentrations from 50 to 2000 pg/mL of antidigoxin. |
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Keywords: | Biosensor Diffraction Immunoassay Microcontact printing Sandwich assay |
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