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Peptide syntheses mediated by Bacillus subtilis protease |
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| Authors: | Toshifumi Miyazawa Shino Masaki Kayoko Tanaka and Takashi Yamada |
| Institution: | (1) Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe, 658-8501, Japan |
| Abstract: | Bacillus subtilis protease (Amano protease N) was examined as a catalyst for peptide bond formation via both the kinetically and thermodynamically controlled approaches. In general, the latter approach proved to be superior to the former, and a series of dipeptide syntheses and several segment condensations were achieved in good to high yields using the immobilized enzyme on Celite in acetonitrile with low water content. |
| Keywords: | Bacillus subtilis protease kinetically controlled approach protease-catalyzed peptide synthesis thermodynamically controlled approach |
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