Human chromosome 21-encoded cDNA clones |
| |
| Authors: | R L Neve G D Stewart P Newcomb M L Van Keuren D Patterson H A Drabkin D M Kurnit |
| |
| Affiliation: | 1. Division of Genetics, The Children''s Hospital, Boston, MA 02115, Tel. (617)735-7240 U.S.A.;2. Eleanor Roosevelt Institute for Cancer Research, University of Colorado Health Science Center, Denver, CO 80262 U.S.A. Tel. (303)394-7152;1. Laboratory of Neurodegenerative Diseases, Centro de Investigación Biomédica, Universidad Autónoma de Chile, Santiago, Chile;2. Centro de Investigación y Estudio del Consumo de Alcohol en Adolescentes (CIIA), Santiago, Chile;3. Department of Anesthesiology and Perioperative Medicine, University of Rochester Medical Center, NY, USA;4. Laboratory of Neurobiology of Aging, Centro de Biología Celular y Biomedicina (CEBICEM), Universidad San Sebastián, Santiago, Chile;1. The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China;2. Department of Neurobiology and Acupuncture Research, Zhejiang Chinese Medical University, Hangzhou, China;1. Neurodegeneration, H. Lundbeck A/S, DK-2500 Valby, Denmark;2. Departments of Neuroscience and Physiology, and Psychiatry, New York University School of Medicine, New York, NY 10016, USA |
| |
| Abstract: | We have employed two strategies to isolate random cDNA clones encoded by chromosome 21. In the first approach, a cDNA library representing expressed genes of WA17, a mouse-human somatic cell hybrid carrying chromosome 21 as its sole human chromosome, was screened with total human DNA to identify human chromosome 21-specific cDNAs. The second approach utilized previously characterized single-copy genomic fragments from chromosome 21 as probes to retrieve homologous coding sequences from a human fetal brain cDNA library. Six cDNA clones on chromosome 21 were obtained in this manner. Two were localized to the proximal long arm of chromosome 21, two to the distal portion of the long arm, and one to the region of 21q22 implicated in the pathology of Down syndrome. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
