PHOSPHOLIPID METABOLISM IN EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS: ACTIVITY OF BRAIN PHOSPHOLIPASE A1, TOWARDS SPECIFICALLY LABELLED GLYCEROPHOSPHOLIPIDS |
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| Authors: | H Woelk K Kanig |
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| Institution: | Nervenklinik und Abteilung für Neurochemie, J. L. W. Thudichum, der Universität des Saarlandes, Homburg/Saar, GFR |
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| Abstract: | —The activity of phospholipase A1 (phosphatide acylhydrolase, EC 3.1.1.4) was measured against phosphatidylcholine, -ethanolamine and -serine, specifically labelled with different fatty acids at either the 1 or 2 position, during experimental allergic encephalomyelitis in rats. In the acute stage of the disease there was a significant increase in enzyme activity in comparison to that from the control animals. The enhanced phospholipase A1 activity was of the-same order of magnitude for all 1,2-diacyl-glycerophospholipids investigated. The phospholipase A1-catalysed release of labelled fatty acids from molecular species of phosphatidylcholine decreased slightly with increasing degree of unsaturation of the fatty acids at the 1-position. The Km-values of the highly purified enzyme were not altered by the demyelinating disorder. The phospholipase A1 activity returned to the control level in the recovery stage. |
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