Treatment with Vitamin D/MOG Association Suppresses Experimental Autoimmune Encephalomyelitis |
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| Authors: | Fernanda Chiuso-Minicucci Larissa Lumi Watanabe Ishikawa Luiza Ayumi Nishiyama Mimura Thais Fernanda de Campos Fraga-Silva Thais Graziela Donegá Fran?a Sofia Fernanda Gon?alves Zorzella-Pezavento Camila Marques Maura Rosane Valerio Ikoma Alexandrina Sartori |
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| Institution: | 1Department of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil;2Laboratório de Citometria de Fluxo—Fundação Dr. Amaral Carvalho, Jaú, São Paulo, Brazil;University of Texas at San Antonio, UNITED STATES |
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| Abstract: | Experimental autoimmune encephalomyelitis (EAE) is an animal model to study multiple sclerosis (MS). Considering the tolerogenic effects of active vitamin D, we evaluated the therapeutic effect of myelin oligodendrocyte glycoprotein (MOG) associated with active vitamin D in EAE development. EAE was induced in female C57BL/6 mice by immunization with MOG emulsified with Complete Freund’s Adjuvant plus Mycobacterium tuberculosis. Animals also received two intraperitoneal doses of Bordetella pertussis toxin. One day after immunization, mice were treated with 0,1μg of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) every other day during 15 days (on days 1, 3, 5, 7, 9, 11, 13 and 15). MOG (150μg) was co-administered on days 3 and 11. The administration of 1,25(OH) 2D3 or MOG determined significant reduction in EAE incidence and in clinical scores. When MOG was associated with 1,25(OH) 2D3 the animals did not develop EAE. Spleen and central nervous system (CNS) cell cultures from this group produced less IL-6 and IL-17 upon stimulation with MOG in comparison to the EAE control group. In addition, this treatment inhibited dendritic cells maturation in the spleen and reduced inflammatory infiltration in the CNS. The association of MOG with 1,25(OH) 2D3 was able to control EAE development. |
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