Modeling the Cellular Mechanisms and Olfactory Input Underlying the Triphasic Response of Moth Pheromone-Sensitive Projection Neurons

In the antennal lobe of the noctuid moth Agrotis ipsilon, most pheromone-sensitive projection neurons (PNs) exhibit a triphasic firing pattern of excitation (E₁)-inhibition (I)-excitation (E₂) in response to a pulse of the sex pheromone. To understand the mechanisms underlying this stereotypical discharge, we developed a biophysical model of a PN receiving inputs from olfactory receptor neurons (ORNs) via nicotinic cholinergic synapses. The ORN is modeled as an inhomogeneous Poisson process whose firing rate is a function of time and is fitted to extracellular data recorded in response to pheromone stimulations at various concentrations and durations. The PN model is based on the Hodgkin-Huxley formalism with realistic ionic currents whose parameters were derived from previous studies. Simulations revealed that the inhibitory phase I can be produced by a SK current (Ca²⁺-gated small conductance K⁺ current) and that the excitatory phase E₂ can result from the long-lasting response of the ORNs. Parameter analysis further revealed that the ending time of E₁ depends on some parameters of SK, Ca²⁺, nACh and Na⁺ currents; I duration mainly depends on the time constant of intracellular Ca²⁺ dynamics, conductance of Ca²⁺ currents and some parameters of nACh currents; The mean firing frequency of E₁ and E₂ depends differentially on the interaction of various currents. Thus it is likely that the interplay between PN intrinsic currents and feedforward synaptic currents are sufficient to generate the triphasic firing patterns observed in the noctuid moth A. ipsilon.