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Dimethylsulfoxide and sodium butyrate enhance the production of recombinant cyclooxygenase 2 in stably transformed Drosophila melanogaster S2 cells |
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| Authors: | Kyung Hwa Chang Jong Hwa Park Youn Hyung Lee Jong Ho Kim Hyung Ok Chun Je Hak Kim In Sik Chung |
| Institution: | (1) Department of Genetic Engineering, Kyung Hee University, Suwon, 449-701, Korea;(2) Plant Metabolism Research Center, Kyung Hee University, Suwon, 449-701, Korea;(3) Institute of Science and Technology, Cheil-Jedang Corporation, Dokpyong, Majang, Ichon, Kyonggi-do, 467-810, Korea;(4) Department of Genetic Engineering; Plant Metabolism Research Center, Kyung Hee University, Suwon, 449-701, Korea |
| Abstract: | Recombinant human cyclooxygenase 2 (Cox 2) was expressed in stably transformed Drosophila melanogaster S2 cells, and was present primarily in the cellular fraction at a molecular weight of 70 to 74 kDa. Recombinant Cox 2 was purified using Ni2+-affinity fractionation to a specific activity of 24 800 U mg–1 protein. The peak level of recombinant Cox 2 production was 1.6  g (107 cells)–1, seven days after induction with 0.5 mM CuSO4. Supplementing the cultures with dimethylsulfoxide or sodium butyrate increased recombinant Cox 2 production by 170% and 86%, respectively. |
| Keywords: | activity cyclooxygenase 2 dimethylsulfoxide Drosophila melanogaster S2 cells purification sodium butyrate |
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