Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells |
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| Authors: | Hasunuma Tomohisa Fukusaki Ei-ichiro Kobayashi Akio |
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| Affiliation: | Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita 565-0871, Japan. |
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| Abstract: | Tobacco suspension culture cell (Nicotiana tabacum, BY2) was transformed with an Aspergillus niger pectin methylesterase (PME; EC 3.1.1.11) cDNA under the control of cauliflower mosaic virus (CaMV) 35S promoter. The transformant indicated a significant rise of PME and the level of methanol in the transformant increased by 28.7% compared to the vector control transformant. This is the first report of methanol overproduction in plant cells by means of genetic engineering. |
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