Calcium Transport by Frankia sp. Strain EAN1pec |
| |
Authors: | Louis S Tisa |
| |
Institution: | (1) Department of Microbiology, University of New Hampshire, Durham, NH 03824-2617, USA , US |
| |
Abstract: | When incubated at 25°C, N2-grown cells of Frankia strain EAN1pec actively accumulated calcium, while NH4Cl-grown cells did not accumulate calcium. When incubated at 0°C, both N2-grown and NH4Cl-grown cells did not actively accumulate calcium. Inhibitors of respiration inhibited calcium accumulation by N2-grown cells at 25°C. Isolated vesicles also accumulated calcium in an energy- and temperature-dependent manner. Two lines
of evidence show that Frankia strain EAN1pec has an active calcium extrusion mechanism. First, NH4Cl-grown cells incubated under deenergizing conditions accumulated calcium. Second, calcium efflux from calcium-loaded cells
required an energy source and was blocked by inhibitors. The results of this study indicate that Frankia strain EAN1pec has two systems for calcium transport: a calcium extrusion system and a developmentally regulated calcium
uptake system.
Received: 1 December 1997 / Accepted: 9 January 1998 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|