Partial purification and some properties of a 5'-nucleotidase from chicken liver with activity toward pyrimidine nucleoside monophosphates

A 5'-nucleotide phosphohydrolase activity, purified about 200-fold from cytosol of chicken liver, prefers as substrates pyrimidine nucleoside monophosphates, particularly the cytidilic forms. This activity, independent by divalent metal ions, shows a pH optimum of 6.2 and an isoelectric point of 5.2.