|
|||||
|
|
Distribution of transfer RNA genes in thePisum sativum chloroplast DNA |
|
| Authors: | NM Chu DR Shapiro KK Oishi KK Tewari |
| Affiliation: | (1) Department of Molecular Biology and Biochemistry, University of California, 92717 Irvine, CA, U.S.A.;(2) Present address: Laboratory of Plant Molecular Biology, The Rockefeller University, 10021 New York, NY, U.S.A.;(3) Present address: Department of Genetics, University of California, 94720 Berkeley, CA, U.S.A. |
| Abstract: | Purified chloroplast tRNAs were isolated fromPisum sativum leaves and radioactively labeled at their 3′ end using tRNA nucleotidyl transferase and α32P-labeled CTP. Pea ctDNA was fragmented using a number of restriction endonucleases and hybridized with thein vitro labeled chloroplast tRNAs by DNA transfer method. Genes for tRNAs have been found to be dispersed throughout the chloroplast genome. A closer analysis of the several hybrid regions using recombinant DNA plasmids have shown that tRNA genes are localized in the chloroplast genome in both single and multiple arrangements. Two dimensional gel electrophoresis of total ct tRNA have identified 36 spots. All of them have been found to hybridize withPisum sativum ctDNA. Using recombinant clones, 30 of the tRNA spots have been mapped inPisum sativum ctDNA. |
| Keywords: | transfer RNA gene localization restriction mapping hybridization chloroplast |
| 本文献已被 SpringerLink 等数据库收录！ | |