A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium |
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Authors: | Biedendieck Rebekka Gamer Martin Jaensch Lothar Meyer Steffen Rohde Manfred Deckwer Wolf-Dieter Jahn Dieter |
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Institution: | Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany. |
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Abstract: | A sucrose-inducible promoter system (P(sacB)) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l(-1)) and the heterologous green fluorescent protein GFP (7.9 mg g(CDW)(-1)). Mutational analysis of B. megaterium P(sacB) allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P(xylA)) for recombinant gene expression in B. megaterium. |
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