A rapid and efficient system of Agrobacterium infection-mediated transient gene expression in rice Oc cells and its application for analysis of the expression and antisense suppression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene |
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| Authors: | Yang H Morita A Matsubayashi Y Nakamura K Sakagami Y |
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| Institution: | Laboratory of Bioactive Natural Product Chemistry, Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Japan. hyang@agr.nagoya-u.ac.jp |
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| Abstract: | A rapid and efficient system for Agrobacterium infection-mediated transient gene expression in rice has been developed. Using this system, transient expression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene was analyzed. The results suggest that the Agrobacterium infection-mediated transient gene expression system is as efficient in rice Oc cells as in tobacco BY-2 cells and might be useful for rapid analysis not only of foreign gene expression, but also of antisense gene suppression. |
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