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Development and characterization of microsatellite loci for lotus (Nelumbo nucifera) |
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| Authors: | Hong-Li Tian Xiao-Qing Chen Jian-Xiu Wang Jian-Hua Xue Jun Wen Grant Mitchell Shi-Liang Zhou |
| Institution: | 1. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing, 100093, China 2. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China 3. Department of Life Sciences, Daqing Normal College, Daqing, 163712, China 4. Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC, 20013-7012, USA 5. Liang Lian Lotus & Waterlily Farm, R. M. B. 824, Bunga, 2550, NSW, Australia |
| Abstract: | This paper reports the development of microsatellite primers for Nelumbo nucifera Gaerten. By screening genomic libraries enriched with 10 kinds of probes, Seventeen polymorphic loci were isolated and primers were designed. Polymorphism of these 17 loci was assessed in 24 individuals. All the 17 loci are polymorphic and the number of alleles ranged from two to seven. Observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.9176 and from 0.2837 to 0.7917 respectively. These microsatellite loci should be useful for studying the genetic diversity of N. nucifera. |
| Keywords: | Nelumbo nucifera Genetic diversity Microsatellite primers SSR |
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