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| 诱导物及前体物对香荚兰细胞悬浮培养产生香兰素的影响 | |
| 引用本文： | 曹孟德,李家儒,王君健.诱导物及前体物对香荚兰细胞悬浮培养产生香兰素的影响[J].植物研究,2000,20(3):266-269. |
| 作者姓名： | 曹孟德 李家儒 王君健 |
| 作者单位： | 1. 河南医科大学博士后流动站, 郑州 450052; 2. 中国科学院武汉水生生物研究所, 武汉 430072; 3. 华中理工大学生物工程系, 武汉 430074 |
| 摘 要： | 未经高温处理的黑曲霉诱导物对香荚兰细胞培养产生香兰素有促进作用;苯丙氨酸有利于香荚兰细胞生长,但对香兰素的合成无影响;阿魏酸则减缓细胞生长,明显促进香兰素的合成,但其浓度不宜高于5mmol/L。 |
| 关 键 词： | 香荚兰 香兰素 悬浮培养 诱导物 前体物 |
| 收稿时间： | 1999-07-29 |
EFFECTS OF ELICITOR AND PRECURSOR ON THE VANILLIN FORMATION IN BATCH SUSPENSION CELL CULTURE OF VANILLA PLANIFOLIA |
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| CAO Meng-de,LI Jia-ru,WANG Jun-jian.EFFECTS OF ELICITOR AND PRECURSOR ON THE VANILLIN FORMATION IN BATCH SUSPENSION CELL CULTURE OF VANILLA PLANIFOLIA[J].Bulletin of Botanical Research,2000,20(3):266-269. | |
| Authors: | CAO Meng-de LI Jia-ru WANG Jun-jian |
| Institution: | 1. Department of Basic Science, Henan Medical University, Zhengzhou 450052; 2. Institute of Hydrobiology, the Chinese Academy of Science, Wuhan 430072; 3. Bioengineering Department, Huazhong University of Science & Technology, Wuhan 430074 |
| Abstract: | The non-autoclaved Aspergillus niger v.Tiegh elicitor could promote vanillin biosynthesis in suspension cell culture of Vanilla planifolia; Phenylalanine was profitable to cell growth of Vanilla planifolia, but did not affect vanillin formation;ferulic acid could slow down cell growth and increase vanillin accumulation,but its concentration should not be higher than 5m mol/L. |
| Keywords: | Vanilla planifolia Vanillin Suspension culture Elicitor Ferulic acid Phenylalanine |
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