Detection of lipopolysaccharides blotted to polyvinylidene difluoride membranes |
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| Authors: | M A Stein S A McAllister K H Johnston D L Diedrich |
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| Affiliation: | Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, New Orleans 70112-1393. |
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| Abstract: | Bacterial lipopolysaccharide (LPS) blotted to polyvinylidene difluoride (PVDF) membranes was detected by a technique adapted from current methodologies used to detect glycoproteins. PVDF-bound LPS was coupled to a hapten and localized on the membrane by Western blotting with an antibody-alkaline phosphatase conjugate specific for the hapten. Immobilon blots could be made reversibly transparent for photography and densitometry. |
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