Electron Tomography of Large, Multicomponent Biological Structures |
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| Authors: | G.A. Perkins C.W. Renken J.Y. Song T.G. Frey S.J. Young S. Lamont M.E. Martone S. Lindsey M.H. Ellisman |
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| Affiliation: | aDepartment of Biology, San Diego State University, San Diego, California, 92182-4614;bNational Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, California, 92093-0608 |
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| Abstract: | Electron tomography is an extremely useful method for deriving three-dimensional structure from electron microscope images. The application of this technique to the reconstruction of large, complex structures such as mitochondria is described in conjunction with several tools for segmentation, measurement, classification, and visualization. In addition, the use of massively parallel computers to perform the tomographic reconstruction efficiently using R-weighted backprojection or iterative techniques is described. |
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