| 黑翅土白蚁踪迹信息素组分数量的柱层析分离和活性测定分析 |
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| 引用本文： | 文平,嵇保中,刘曙雯,冀仕琳,刘佳佳. 黑翅土白蚁踪迹信息素组分数量的柱层析分离和活性测定分析[J]. 昆虫知识, 2013, 0(6): 1622-1627 |
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| 作者姓名： | 文平 嵇保中 刘曙雯 冀仕琳 刘佳佳 |
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| 作者单位： | [1]南京林业大学森林资源与环境学院,南京210037 [2]南京中山陵园管理局,南京210014 [3]江苏省农用激素工程技术研究中心,常州213022 |
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| 基金项目： | 高等学校博士学科点专项科研基金项目（20123204110001）;江苏省自然科学基金项目（BK2012816）;南京市建委中山陵园管理局资助课题（200409）;江苏高校优势学科建设工程资助项目（164010641）. |
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| 摘 要： | 本文分析了在活性限浓度进行生物活性测定时,黑翅土白蚁Odontotermes formosanu Shiraki对踪迹信息素的行为反应特点以及使用不同踪迹线载体对踪迹信息素活性的影响。并设计标准生物活性测试方法,测定了采用柱层析方法分离的黑翅土白蚁踪迹信息素各分离馏分的活性,发现黑翅土白蚁踪迹信息素用22%乙酸乙酯/正己烷洗脱液等度洗脱分离后的各馏分中包含两个强活性区段,推测黑翅土白蚁踪迹信息素至少包含2个有轻微极性差异的组分。
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| 关 键 词： | 黑翅土白蚁 踪迹信息素 柱层析 活性组分 |
Liquid chromatography and bioassay analysis of components in the trail pheromone of the black-wing subterranean termite Odontotermes formosanus Shiraki |
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| WEN Ping,JI Bao-Zhong LIU Shu-Wen JI Shi-Lin LIU Jia-Jia. Liquid chromatography and bioassay analysis of components in the trail pheromone of the black-wing subterranean termite Odontotermes formosanus Shiraki[J]. Entomological Knowledge, 2013, 0(6): 1622-1627 |
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| Authors: | WEN Ping JI Bao-Zhong LIU Shu-Wen JI Shi-Lin LIU Jia-Jia |
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| Affiliation: | 1. College of Forest Resource and Environment, Nanjing Forestry University, Nanjing 210037, China 2. Administration Bureau of Dr Sun Yat-Sen＇ s Mausoleum, Nanjing 210014, China; 3. Jiangsu Agroehem Laboratory, Changzhou 213022, China) |
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| Abstract: | By working at the threshold active concentration, the characteristic behavioral reactions of the black-winged subterranean termite Odontotermes formosanus Shiraki to extracts of trail pheromone components were analyzed in trail- following bioassays, including the effect of different testing substrates. Based on the results, a standard bioassay method was used to test the fractions from isocratic elution （22% EtOAc/n-hexane） of pheromone extract on a silica gel column. Results show that two active peaks exist in the fractions, which indicate that the trail pheromone of O. formosanus consists of at least two components. |
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| Keywords: | Odontotermes formosanus trail pheromone liquid chromatograph active components |
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