Functional characterisation of the Drosophila cg6568 gene in host defence against Mycobacterium marinum |
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| Affiliation: | 1. Department of Microbiology, Chungnam National University School of Medicine, Daejeon, South Korea;2. Pulmonary Medicine, Chungnam National University School of Medicine, Daejeon, South Korea;3. Infection Biology, Chungnam National University School of Medicine, Daejeon, South Korea;4. Molecular Mechanism of Antibiotics, Division of Life Science, Research Institute of Life Science, Gyeongsang National University, Jinju, Gyeongnam, South Korea;5. Biomedical Research Institute, Chungnam National University Hospital, Daejeon, South Korea;6. Department of Biological Sciences, Konkuk University, Seoul, South Korea;1. Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Guizhou Province, China;2. Z-BioMed, Rockville, MD, USA;1. Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 500-712, South Korea;2. Department of Chemistry, Yonsei University, Seoul 120-749, South Korea;1. Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, College Station, TX 77843, USA;1. Department of Biology, Volen National Center for Complex Systems and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 02454-9110, USA;2. Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA |
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| Abstract: | Mycobacterium marinum is a pathogenic mycobacterial species closely related to Mycobacterium tuberculosis. In this study, we established a mycobacterial infection model of Drosophila melanogaster to characterize the role played by cg6568, a homolog of the human cathelicidin gene, in the innate defense against infection. Drosophila cg6568 was expressed at various levels during all developmental stages, and the expression levels were modulated by M. marinum in a time-dependent manner. 20-hydroxyecdysone induced Drosophila cg6568 transcription both in vitro and in vivo. Using flies expressing cg6568 RNAi, we found that cg6568 was essential both for D. melanogaster survival and the exertion of antimicrobial effects during M. marinum infection. Thus, we named the gene product a cathelicidin-like antimicrobial protein of D. melanogaster (dCAMP). Our results indicate that dCAMP is crucial in terms of the innate D. melanogaster defense during M. marinum infection. |
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| Keywords: | Antimicrobial peptide Innate immunity Cathelicidin |
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