Evaluation of MDCK Cell-Derived Influenza H7N9 Vaccine Candidates in Ferrets |
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| Authors: | Min-Yuan Chia Alan Yung-Chih Hu Yu-Fen Tseng Tsai-Chuan Weng Chia-Chun Lai Jun-Yang Lin Po-Ling Chen Ya-Fang Wang Sin-Ru Chao Jui-Yuan Chang Yi-Shiuh Hwang Chia-Tsui Yeh Cheng-Ping Yu Yee-Chun Chen Ih-Jen Su Min-Shi Lee |
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| Institution: | 1. National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.; 2. Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.; University of Georgia, UNITED STATES, |
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| Abstract: | Avian-origin influenza A (H7N9) viruses emerged as human pathogens in China in early 2013 and have killed >100 persons. Influenza vaccines are mainly manufactured using egg-based technology which could not meet the surging demand during influenza pandemics. In this study, we evaluated cell-based influenza H7N9 vaccines in ferrets. An egg-derived influenza H7N9 reassortant vaccine virus was adapted in MDCK cells. Influenza H7N9 whole virus vaccine antigen was manufactured using a microcarrier-based culture system. Immunogenicity and protection of the vaccine candidates with three different formulations (300μg aluminum hydroxide, 1.5μg HA, and 1.5μg HA plus 300μg aluminum hydroxide) were evaluated in ferrets. In ferrets receiving two doses of vaccination, geometric mean titers of hemagglutination (HA) inhibition and neutralizing antibodies were <10 and <40 for the control group (adjuvant only), 17 and 80 for the unadjuvanted (HA only) group, and 190 and 640 for the adjuvanted group (HA plus adjuvant), respectively. After challenge with wild-type influenza H7N9 viruses, virus titers in respiratory tracts of the adjuvanted group were significantly lower than that in the control, and unadjuvanted groups. MDCK cell-derived influenza H7N9 whole virus vaccine candidate is immunogenic and protective in ferrets and clinical development is highly warranted. |
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