Sperm ultrastructure of shrimps from the family Penaeidae (Crustacea: Dendrobranchiata) in a phylogenetic context |
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| Affiliation: | 1. Laboratório de Morfologia de Invertebrados (IML), Departamento de Biologia Aplicada, CAUNESP and IEAMar, Universidade Estadual Paulista (UNESP), FCAV, Jaboticabal, SP, Brazil;2. Laboratório de Bioecologia e Sistemática de Crustáceos (LBSC), Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo (USP), Av. Bandeirantes, 3900, 14040-901, Ribeirão Preto, SP, Brazil;3. Laboratório de Carcinologia, Departamento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Distrito de Rubião Júnior, S/N, 18618-970, Botucatu, SP, Brazil;4. Laboratório de Biologia de Camarões Marinhos e de Água Doce (LABCAM), Departamento de Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista (UNESP), Av. Luiz Edmundo Carrijo Coube n° 14-01, Bairro Vargem Limpa, 17033-360, Bauru, SP, Brazil;5. Instituto de Ciências Biológicas (ICB), Universidade Federal de Goiás, Av. Esperança, s/n, Campus Samambaia (Campus II) ICB 01: sala -18, 74690-900, Goiânia, GO, Brazil;1. Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand;2. Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12121, Thailand;3. Mahidol University, Nakhonsawan Campus, Nakhonsawan 60130, Thailand;4. Faculty of Science, Health and Education, University of the Sunshine Coast, QLD 4558, Australia;5. Department of Anatomy, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;6. Pro Vice-Chancellor''s Office, Faculty of Science, Engineering and Built Environment, Deakin University, Locked Bay 20000, Geelong, VIC 3220, Australia;1. Hormozgan University, Faculty of Science, Bandar abbas, Iran;2. Chabhar Maritime University, Faculty of Marine Sciences, Chabhar, Iran;1. UNESP – Universidade Estadual Paulista, 13506-900 Rio Claro, SP, Brazil;2. Invertebrate Morphology Laboratory (IML), Departamento de Biologia Aplicada, CAUNESP and IEAMar, UNESP – Universidade Estadual Paulista, 14884-900 Jaboticabal, SP, Brazil;1. Department of Life Science, University of Siena, Italy;2. Institute of Mediterranean Forest Ecosystem, HAO DEMETER, Athens, Greece;3. Consiglio per la Ricerca in Agricoltura e l''analisi dell''economia agraria, Centro di Ricerca per l''Agrobiologia e la Pedologia (CREA-ABP), Firenze, Italy |
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| Abstract: | We describe the sperm ultrastructure of six penaeid species, including at least one member of each tribe (Penaeini, Parapenaeini and Trachypenaeini). Fragments of the vas deferens of the Penaeidae Farfantepenaeus brasiliensis, Farfantepenaeus paulensis, Litopenaeus schmitti, Parapenaeus americanus, Rimapenaeus constrictus and Xiphopenaeus kroyeri were fixed and processed according to the routine for transmission electron microscopy. The morphological results were contextualized in an evolutionary perspective using molecular markers for the phylogenetic reconstruction of this group. A phylogram was proposed by Bayesian inference based on 1007 bp of 33 sequences of the combined genes (16S rDNA and COI mtDNA) from 27 dendrobranchiate specimens. Our findings show that morphological differences in the sperm ultrastructures of members among the tribes of Penaeidae can be used as a baseline to understand their evolutionary relationships. Individuals from the Penaeini tribe show plesiomorphic characteristics in the sperm ultrastructure compared to the Trachypenaeini tribe from which they were derived, such as shrimp from family Sicyoniidae. The morphological complexity of the sperm of the different penaeid members corroborated with the genetic phylogeny, which showed different clades for each tribe and the close relationship with Sicyoniidae. The sperm features of the selected species studied here reflected their evolutionary history. These features confirm the previous phylogenetic hypothesis and question the monophyly of Penaeidae, which should be verified in the future with a more complete set of representative members of each tribe. |
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| Keywords: | Sperm ultrastructure Molecular phylogeny Transmission electron microscopy Bayesian inference Decapoda |
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