BmNPV-miR-415 up-regulates the expression of TOR2 via Bmo-miR-5738 |
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Institution: | 1. Jiangsu University of Science and Technology, Zhenjiang City 212018, Jiangsu Province, PR China;2. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang City 471003, Henan Province, PR China;3. Dezhou College, Dezhou City 253023, Shandong Province, PR China;4. The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang City 212018, Jiangsu Province, PR China |
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Abstract: | MicroRNAs (miRNAs) have emerged as key players in host–pathogen interaction and many virus-encoded miRNAs have been identified (computationally and/or experimentally) in a variety of organisms. A novel Bombyx mori nucleopolyhedrosis virus (BmNPV)-encoded miRNA miR-415 was previously identified through high-throughput sequencing. In this study, a BmNPV-miR-415 expression vector was constructed and transfected into BmN cells. The differentially expressed protein target of rapamycin isoform 2 (TOR2) was observed through two-dimensional gel electrophoresis and mass spectrometry. Results showed that TOR2 is not directly a target gene of BmNPV-miR-415, but its expression is up-regulated by BmNPV-miR-415 via Bmo-miR-5738, which could be induced by BmNPV. |
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Keywords: | 3′ UTR MicroRNAs BmNPV TOR2 |
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