Single step purification of polysaccharides using immobilized jackfruit lectin as affinity adsorbent

An -d-galactosyl-binding lectin fromArtocarpus integrifolia (jackfruit) seeds has been coupled to cyanogen bromide-activated Sepharose 4B. Purification of three galactomannans from fenugreek, guar andPoinciana pulcherrima seeds, a galactoglucomannan fromCrotalaria saltiana seed and a polysaccharide from the albumin gland of the snailLittorina littorea has been achieved by affinity chromatography on a lectin-Sepharose column. The recovery of the polysaccharides absolutely devoid of protein is about 40%.Presented at the 8th International Lectin Meeting, La Paz, 1986.